Recipe

Freezing Medium for Feeder-Free Culture (1×)

Reagent Final concentration
DMSO 10%
KnockOut Serum Replacement (KSR) (Invitrogen) 90%
Prepare fresh for each use and keep on ice.
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  1. doi:10.1101/pdb.rec095901 Cold Spring Harb Protoc 2017: pdb.rec095901- © 2017 Cold Spring Harbor Laboratory Press

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