Pancreatin/Trypsin Solution

Recipe

Reagent	g/20 mL	Final concentration
Pancreatin	0.50	2.5%
Trypsin	0.10	0.5%
Polyvinylpyrrolidone (optional)	0.10	0.5%
Make in Ca²⁺/Mg²⁺-free Tyrode Ringer's saline.
The suspension will be difficult to filter-sterilize through a 0.45-µm Millipore filter without low-speed centrifugation or prefiltering through a Whatman No. 1 filter. Store sterile in small aliquots at −20°C.
Modified from Levak-Svajger et al. (1969).

REFERENCES

1. Levak-Svajger B,
2. Svajger A,
3. Skreb N
Levak-Svajger B, Svajger A, Skreb N. 1969. Separation of germ layers in presomite rat embryos. Experientia 25: 1311–1312.
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