Collagenase Type IV (1 mg/mL)
Dissolve 0.5 g of collagenase type IV (Invitrogen 17104-019) in 500 mL of EpiSC medium. Filter, aliquot in 10 mL, and freeze at −80°C. Thaw each aliquot as needed and store it at 4°C (do not refreeze).
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