Recipe

CDM + FGF2 + ActA Medium

Reagent Stock concentration Final concentration Amount
FGF2 stock solution 10 µg/mL 12 ng/mL  12 µL
Activin A stock solution 10 µg/mL 20 ng/mL  20 µL
Chemically defined medium (CDM) 10 mL
Store at 4°C and use within 1 wk.
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  1. doi:10.1101/pdb.rec095950 Cold Spring Harb Protoc 2017: pdb.rec095950- © 2017 Cold Spring Harbor Laboratory Press
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