Protocol

Integrating piggyBac Transposon Transgenes into Mouse Fibroblasts by Electroporation

  1. Andras Nagy

Abstract

Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.

Footnotes

  • From the Manipulating the Mouse Embryo collection, edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, and Andras Nagy.

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  1. doi:10.1101/pdb.prot092601 Cold Spring Harb Protoc 2017: pdb.prot092601- © 2017 Cold Spring Harbor Laboratory Press
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