Recipe

Lysis Buffer for β-Galactosidase Assays

Tris–Cl (pH 7.8)   0.1 m
Triton X-100 0.5% (v/v)
The detergent Nonidet P-40 at 0.125% (v/v) can be substituted for Triton X-100 in the lysis buffer.
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  1. doi:10.1101/pdb.rec096602 Cold Spring Harb Protoc 2017: pdb.rec096602- © 2017 Cold Spring Harbor Laboratory Press
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