Labeling Medium for [35S]Methionine Labeling
| Reagent | Volume per 100 mL of solution | Final concentration |
|---|---|---|
| Methionine-free DMEM (1×) | 9–10 mL (depending on serum concentration) | 1× |
| Dialyzed calf serum | 0–1 mL | 0%–10% as required |
| l-Glutamine (100×, 200 mm) | 0.1 mL | 1× |
| HEPES-KOH (pH 7.4, 1 m) | 250 µL | 25 mm |
| [35S]Methionine (10 mCi/mL) | 0.3–2 mL | 300 µCi/mL to 2 mCi/mL |
| Prepare fresh under sterile conditions. Use dialyzed serum if cells require serum for survival. Warm to 37°C and add [35S]methionine last in case mistakes are made in the medium preparation. | ||
