Earle's Balanced Salt Solution (EBSS, 10×)
100 mL Earl's Balanced Salt Solution (EBSS) (10×) (Sigma E7510)
0.2 m NaHCO3
0.15 m NaOH
To prepare 150 mL of 10× EBSS, combine the above reagents and sterilize through 0.22-micron filter. Store aliquots at −20°C.
