Objective choice and image processing. Images of gfp–atb2 myo52–mCherry cells captured on the same widefield system with the same camera and the indicated lenses (all from the same manufacturer) either with or without deconvolution. Arrows highlight spatial resolution allowed by WF microscopy that allows the researcher to discern that microtubules (green) fit cleanly into gaps between foci of the myosin V (magenta). Scale bars: 5 µm.