Corrigendum: Purification of Endothelial Cells from Rodent Brain by Immunopanning
Cold Spring Harb Protoc 2014; doi: 10.1101/pdb.prot074963
When this article was first published, the recipe entitled “T3 Stock (4 µg/mL),” which originally appeared in Dugas and Emery (2013) and Emery and Dugas (2013), contained errors. The quantity and concentration of thyroid hormone (T3) in Step 1 of the recipe were incorrectly listed as 4 µg and 0.8 µg/100 µL, respectively. The quantity of T3 should be 4 mg, and the concentration should be 0.8 mg/100 µL. The authors of the original recipe apologize for these errors. A corrected version of the recipe is below. In addition, the HTML version of the recipe (doi: 10.1101/pdb.rec075507) and the PDF versions of all three articles have been amended accordingly.
T3 Stock (4 µg/mL)-
1. Dissolve 4 mg of thyroid hormone (T3; Sigma-Aldrich T6397) in 500 µL of 1 n NaOH to prepare a solution of 0.8 mg/100 µL.
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2. Add 75 µL of the T3 solution from Step 1 to 150 mL of Dulbecco's phosphate-buffered saline (D-PBS; Invitrogen 14287-080).
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3. Filter solution through a filter-sterilization unit, discarding the first 10 mL.
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4. Aliquot (e.g., 200 µL/tube) and then store at −20°C.
