Protocol

Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection

  1. Narendra Wajapeyee

Abstract

Methylation-specific polymerase chain reaction (MS-PCR) is a more rapid way to detect changes in DNA methylation than is bisulfite sequencing. In addition, by incorporating some basic automation, samples can be prepared and analyzed in a 96-well plate format. The method can be used either quantitatively (qRT-PCR-based MethyLight) or qualitatively (using agarose gels) to detect changes in DNA methylation; both are described in this protocol.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. Published in Advance November 16, 2016, doi: 10.1101/pdb.prot094847 Cold Spring Harb Protoc 2017: pdb.prot094847- © 2017 Cold Spring Harbor Laboratory Press
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