Nonidet P-40 Lysis Buffer
| Reagent | Final concentration |
|---|---|
| NaCl | 150 mm |
| Nonidet P-40 | 1% |
| Tris (pH 8.0) | 50 mm |
| Prepared Nonidet P-40 lysis buffer should be aliquoted and stored at −20°C. Add protease and/or phosphatase inhibitors to a thawed aliquot before immediate use. Discard and do not freeze again. | |
