Lysis Buffer for Animal Tissues
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| KCl (1 m) | 10 mL | 100 mm |
| Glycerol | 10 mL | 10% |
| Tris–HCl (1 m, pH 8.0) | 2 mL | 20 mm |
| MgCl2 (1 m) | 0.5 mL | 5 mm |
| Tween 20 (10%) | 1 mL | 0.1% |
| ddH2O | Add to 100 mL | |
| Store at room temperature. Aliquot a sufficient volume required for tissue lysis, chill on ice, and add protease and/or phosphatase inhibitors (0.2 mm PMSF, 10 mm β-mercaptoethanol, 1 mm sodium vanadate, 10 mm NaF) before immediate use. | ||
