Wash Buffer for Immunoprecipitation
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| NaCl (1 m) | 15 mL | 150 mm |
| EDTA | 0.2 mL | 1 mm |
| Triton X-100 (10%) | 1 mL | 1% |
| Tris (50 mm, pH 7.4) | 20 mL | 10 mm |
| ddH2O | Add to 100 mL | |
| Store at 4°C. Add protease inhibitor cocktail immediately before use. | ||
