Recipe

Hybridization Buffer (HB) for Xenopus Embryos

Reagent Final concentration
Formamide    50%
Saline–sodium citrate (SSC)     5×
Torula yeast RNA  1 mg/mL
Heparin 100 µg/mL
Denhart's     1×
Tween 20    0.1%
EDTA    5 mm
Prepare in sterile milliQ H2O. Store at −20°C.
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  1. doi:10.1101/pdb.rec102780 Cold Spring Harb Protoc 2017: pdb.rec102780- © 2017 Cold Spring Harbor Laboratory Press
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