Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding)
Abstract
This protocol demonstrates how to use MACS software to call peaks in a ChIP-seq data set. MACS is one of the most widely used peak-calling programs. The protocol describes how to run MACS in a UNIX environment and on Galaxy and how to extract the sequences of ±50 bp around the summits of the 500 MACS peaks with the most significant P values.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
