| Yeast nitrogen base without amino acids (Difco) |
6.7 g/L |
| Adenine sulfate |
5 mg/L |
| Dextrose |
20 g/L |
| Agar (for solid medium) |
20 g/L |
| Sorbitol |
1 m |
| L-Tyrosine |
50 mg/L |
| L-Arginine HCl |
50 mg/L |
| L-Aspartic acid |
80 mg/L |
| L-Histidine HCl |
20 mg/L |
| L-Isoleucine |
50 mg/L |
| L-Lysine HCl |
50 mg/L |
| L-Methionine |
20 mg/L |
| L-Phenylalanine |
50 mg/L |
| L-Threonine |
100 mg/L |
| L-Tryptophan |
50 mg/L |
| Uracil |
20 mg/L |
| L-Valine |
140 mg/L |
| Adenine hemisulphate |
0.1 mg/L |
| Dissolve the amino acid L-tyrosine first. (When it is added according to its alphabetical position it will not go into solution.)
Autoclave. For solid medium, add 20 mL to each Petri plate. Store at 4°C (this is stable for at least 6 mo).
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