Recipe

STC Buffer

Reagent Final concentration
Sorbitol   1 m
Tris-HCl (pH 7.4) 10 mm
CaCl2 10 mm
Store at 4°C (this is stable for at least 6 mo).
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  1. doi:10.1101/pdb.rec090423 Cold Spring Harb Protoc 2017: pdb.rec090423- © 2017 Cold Spring Harbor Laboratory Press

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