STC Buffer
| Reagent | Final concentration |
|---|---|
| Sorbitol | 1 m |
| Tris-HCl (pH 7.4) | 10 mm |
| CaCl2 | 10 mm |
| Store at 4°C (this is stable for at least 6 mo). | |
- © 2017 Cold Spring Harbor Laboratory Press
| Reagent | Final concentration |
|---|---|
| Sorbitol | 1 m |
| Tris-HCl (pH 7.4) | 10 mm |
| CaCl2 | 10 mm |
| Store at 4°C (this is stable for at least 6 mo). | |