Amyloid-Prion Resuspension Buffer
| Reagent | Final concentration |
|---|---|
| Tris-HCl (pH 7.4) | 50 mm |
| NaCl | 100 mm |
| Sodium dodecyl sulfate (SDS) | 2% |
| Dithiothreitol (DTT) | 5 mm |
| Glycerol | 5% |
| Complete protease inhibitors with EDTA (Roche) | 1× |
| Use buffer immediately, or prepare and keep it without DTT at 4°C for several weeks. Add fresh DTT immediately before use. | |
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