Recipe

Na3VO4 Stock Solution

Prepare a stock solution of 200 mm sodium orthovanadate (Na3VO4). Adjust the pH of the 200 mm stock solution to 10 using 1 n NaOH or 1 n HCl. (At a pH of 10 the solution should be yellow.) Boil until the solution clears (~10 min). Cool to room temperature. Readjust to pH 10. Repeat cycles of boiling and pH adjustment until the solution remains clear at pH 10. Store in aliquots at −20°C.

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  1. doi:10.1101/pdb.rec092940 Cold Spring Harb Protoc 2017: pdb.rec092940- © 2017 Cold Spring Harbor Laboratory Press

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