Tris Immunoprecipitation Buffer

50 mm Tris

1 mm Ethylenediaminetetraacetic acid (EDTA)

20 mm Na-β-glycerophosphate

0.1 mm Na₃VO₄ (diluted from 200 mm Na₃VO₄ stock solution)

100 mm NaCl

50 mm NaF

1 mm phenylmethylsulfonyl fluoride (PMSF)

1 mm dithiothreitol (DTT)

0.1% NP-40

Protease inhibitor cocktail

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