Dissection Solution
| Reagent | Final concentration | Amount (for 500 mL) |
|---|---|---|
| Sucrose (Fluka 84100) | 248 mm | 40 g |
| NaHCO3 (Fluka 31437) | 26 mm | 1.09 g |
| d-glucose (Fluka 49152) | 10 mm | 0.9 g |
| KCl (1 m; Fluka 60129) | 4 mm | 2 mL |
| MgCl2 (1 m; Fluka 63020) | 5 mm | 2.5 mL |
| CaCl2 (1 m; Fluka 21114) | 1 mm | 0.5 mL |
| Phenol red (0.5%; Riedel‐De Haen 32662) | 0.001% | 1 mL |
| Ultrapure H2O | – | to 500 mL |
| Kynurenic acid (100 mm; Fluka 61260) | 2 mm | 1 mL in 50 mL dissection solution |
| After mixing all ingredients except the kynurenic acid, check the osmolality (it should be 310–320 mOsm/kg) and the color (it should be red-magenta, indicating a pH >8). Filter-sterilize (0.2 µm pore size) and store at 4°C in 50-mL aliquots. Prepare a 100 mm stock solution of kynurenic acid and store at −20°C in 1-mL aliquots. Just before use, thaw and vortex one aliquot of kynurenic acid and add to 50 mL of dissection solution. | ||
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