Slice Culture Transduction Solution
| Reagent | Final concentration | Amount (for 500 mL) |
|---|---|---|
| NaCl (Sigma-Aldrich S5150) | 145 mm | 4.23 g |
| HEPES (Sigma-Aldrich H4034) | 10 mm | 1.19 g |
| d-glucose (Fluka 49152) | 25 mm | 2.25 g |
| KCl (1 m; Fluka 60129) | 2.5 mm | 1.25 mL |
| MgCl2 (1 m; Fluka 63020) | 1 mm | 0.5 mL |
| CaCl2 (1 m; Fluka 21114) | 2 mm | 1 mL |
| Adjust pH to 7.4 with NaOH. After mixing all ingredients, check the osmolality—it should be 310–320 mOsm/kg. Filter-sterilize (0.2-µm pore size) and store at 4°C. | ||
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