Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome
- 1Daniel Rutherford Building G.24, School of Biological Sciences, University of Edinburgh, The King’s Buildings, Edinburgh EH9 3BF, United Kingdom;
- 2MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, People’s Republic of China
- ↵3Correspondence: yizhi.cai{at}ed.ac.uk; jbdai{at}tsinghua.edu.cn
Abstract
De novo DNA synthesis is one of the key enabling technologies for synthetic biology. Methods for large-scale DNA synthesis, in particular, have transformed many facets of life science research, supporting new discoveries in biology through the design of novel synthetic biological systems. This protocol describes in detail the methods currently being used to synthesize and assemble large pieces of DNA for the synthetic yeast genome project. The protocol includes instructions for building block synthesis as well as chunk assembly, each of which can be used as a stand-alone procedure to generate a synthetic DNA of interest.
