Low-Salt LB Solid Medium Containing Kanamycin and X-gal
10 g Bacto-tryptone
5 g Bacto-yeast extract
5 g NaCl
15 g Agar
Combine the above ingredients in 1 L of ddH2O and autoclave to sterilize. Cool medium to pouring temperature. Add 1 mL of filtered kanamycin stock solution (40 mg/mL in ddH2O; 1000×) and 2 mL of 20% X-gal stock solution (20 mg/mL in dimethyl formamide, 500×).
