ISO Buffer (5×)
| 1 m Tris–HCl (pH 7.5) (Invitrogen 15567-027) | 3000 µL |
| 1 m MgCl2 (Sigma-Aldrich M1028) | 300 µL |
| 100 mm dGTP | 60 µL |
| 100 mm dTTP | 60 µL |
| 100 mm dATP | 60 µL |
| 100 mm dCTP | 60 µL |
| 1 m DTT (Sigma-Aldrich D9779) | 300 µL |
| PEG-8000 (USB Affymetrix 19959) | 1.5 g |
| 100 mm NAD (Sigma-Aldrich N1511) | 300 µL |
| H2O | to 6 mL |
| Combine the above ingredients. Store 40-µL aliquots at −20°C. | |
