Recipe

Bead Wash Buffer D

10% 2-propanol

10% ethanol

200 mm NaCl

0.2% sodium dodecyl sulfate

100 mm Tris

8 m urea

Adjust pH to 5.0. Prepare within a few days of the experiment and store at room temperature (20°C–25°C).

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  1. doi:10.1101/pdb.rec092841 Cold Spring Harb Protoc 2017: pdb.rec092841- © 2017 Cold Spring Harbor Laboratory Press
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