Modified TAP Lysis Buffer
1 mm EDTA
10% (v/v) glycerol
20 mm HEPES, pH 7.5
150 mm NaCl
0.8% (v/v) NP-40
1 mm phenylmethylsulfonyl fluoride
1× Complete Protease Inhibitor Cocktail, EDTA-free (11873580001, Roche)
Prepare fresh before use (recommended).
