Topic Introduction

Elementary Protein Analysis in Schizosaccharomyces pombe

  1. Iain M. Hagan1
  1. CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom
  1. 1Correspondence: Agnes.Grallert{at}cruk.manchester.ac.uk; Iain.Hagan{at}cruk.manchester.ac.uk

Abstract

Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability make Schizosaccharomyces pombe an excellent model organism in which to use mass spectrometry to conduct proteome-wide approaches. Here we discuss issues encountered during the analysis of fission yeast protein preparations.

Footnotes

  • From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.

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