Isolation of High-Molecular-Weight DNA Using Organic Solvents
Abstract
Perhaps the most basic of all procedures in molecular cloning is the purification of nucleic acids. The key step, the removal of proteins, can often be performed simply by extracting aqueous solutions of nucleic acids with phenol:chloroform and chloroform.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
