Protocol

Cryopreserving and Thawing Spermatogonial Stem Cells

Abstract

Spermatogonial stem cells (SSCs) can be cryopreserved and stored in liquid nitrogen, using relatively standard cryopreservation techniques. At a subsequent time, they can be thawed for subsequent culture and expansion and then transplanted to generate spermatogenesis.

Footnotes

  • From the Manipulating the Mouse Embryo collection, edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, and Andras Nagy.

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