SD + URA + 5-FOA Medium
-
1. Dissolve 6.7 g of yeast nitrogen base with ammonium sulfate and without amino acids, 1 g of 5-fluoroortic acid (5-FOA), 20 g of dextrose, and 50 mg of uracil in 500 mL of dH2O. If necessary, add other nutritional supplements, such as drop-out mixtures. Heat to 65°C to dissolve all components; adjust to pH 3.5–4; and filter-sterilize.
-
2. Add 20 g of Bacto agar to 500 mL of dH2O, and autoclave in a flask with a volume ≥2 L.
-
3. Add the filter-sterilized solution to autoclaved agar, mix thoroughly, and pour the medium into Petri dishes.
-
This recipe was adapted from Boeke et al. (1987).
-
