Protocol

Library Quantification Using PicoGreen Fluorometry

  1. W. Richard McCombie

Abstract

This protocol describes the quantification of DNA using PicoGreen and a fluorometer to determine the concentration of DNA sample for downstream processing. Because dye-based methods will not detect degraded or short DNA fragments, PicoGreen requires DNAs ≥50 bp, but can less reliably detect fragments as small as 20 bp.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. Published in Advance November 1, 2016, doi: 10.1101/pdb.prot094722 Cold Spring Harb Protoc 2017: pdb.prot094722- © 2017 Cold Spring Harbor Laboratory Press
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