Recipe

Neurobasal + B27 Medium

Reagent Amount
B27 supplement (Invitrogen 17504-044)  2 mL
l-Glutamine (200 mm) (Sigma-Aldrich G5763 or Invitrogen 25030)  1 mL
Neurobasal Medium (Invitrogen 21103-049) 98 mL
Store at 4°C and use within 1 mo.
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  1. doi:10.1101/pdb.rec096081 Cold Spring Harb Protoc 2017: pdb.rec096081- © 2017 Cold Spring Harbor Laboratory Press
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