Recipe

N2B27 Medium

Reagent Amount
DMEM/F12 + N2 medium 25 mL
Neurobasal + B27 medium 25 mL
β-Mercaptoethanol (100 mm)  50 µL
The medium without LIF and inhibitors is stored at 4°C and used within 1 mo.
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  1. doi:10.1101/pdb.rec096131 Cold Spring Harb Protoc 2017: pdb.rec096131- © 2017 Cold Spring Harbor Laboratory Press
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