Denaturation Buffer
6 m urea
2 m thiourea
1% n-Octylglucoside (w/v)
Combine the reagents listed above in 10 mm HEPES buffer (pH 8.0) made with high-purity, deionized H2O (i.e., Milli-Q). Store frozen at −20°C.
6 m urea
2 m thiourea
1% n-Octylglucoside (w/v)
Combine the reagents listed above in 10 mm HEPES buffer (pH 8.0) made with high-purity, deionized H2O (i.e., Milli-Q). Store frozen at −20°C.