Recipe

SCX Solvent B

5 mm potassium dihydrogen phosphate

30% acetonitrile (ACN)

350 mm potassium chloride

Combine the reagents listed above in high-purity, deionized H2O (i.e., Milli-Q). Acidify with trifluoroacetic acid (TFA) to pH 2.7. Store up to 4 wk at room temperature.

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  1. doi:10.1101/pdb.rec092551 Cold Spring Harb Protoc 2017: pdb.rec092551- © 2017 Cold Spring Harbor Laboratory Press
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