EMM2-LowAmm
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Ammonium chloride | 0.32 g | 6 mm |
| Potassium hydrogen phthalate | 3 g | 14.7 mm |
| Na2HPO4 | 2.2 g | 15.5 mm |
| Glucose | 20 g | 111 mm |
| Salt stock (50×) <R> | 20 mL | 1× |
| Vitamins (1000×) <R> | 1 mL | 1× |
| Minerals (10,000×) <R> | 0.1 mL | 1× |
| H2O | to 1 L | |
| Prepare 1 L of EMM2-LowAmm by combining the reagents above. Sterilize by autoclaving at 10 psi for 10 min. (Low pressure and a short cycle is essential to avoid caramelization of glucose and breakdown of vitamins and minerals.) Store at 4°C. | ||
