Protocol

Mating-Type Determination in Schizosaccharomyces pombe

  1. Geneviève Thon2
  1. 1Department of Biosciences and Nutrition, Karolinska Institute, Stockholm SE-141 83, Sweden;
  2. 2Department of Biology, University of Copenhagen, Copenhagen DK-2200, Denmark
  1. 3Correspondence: karl.ekwall{at}ki.se

Abstract

Here we describe how mating-type tests are conducted in Schizosaccharomyces pombe. Two methods can be employed: matings with h and h+ tester strains and polymerase chain reaction (PCR) for mat1 content.

Footnotes

  • From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.

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