Protocol

Solution-Phase Exome Capture

  1. W. Richard McCombie

Abstract

This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. A procedure is also provided for magnetic AMPure bead cleanup.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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This Article

  1. Published in Advance November 1, 2016, doi: 10.1101/pdb.prot094680 Cold Spring Harb Protoc 2017: pdb.prot094680- © 2017 Cold Spring Harbor Laboratory Press
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