Recipe

Potassium Acetate Medium (Liquid or Solid)

Reagent Quantity (for 1 L)
Potassium acetate   16 g
Yeast extract 1.76 g
Dextrose  0.4 g
COM drop-out powder  0.7 g
Agar (for solid medium only)   20 g
Dissolve the potassium acetate, yeast extract, and dextrose in 800 mL of distilled water while stirring continuously. Adjust the pH to 7.0 with 1 m HCl or 2.5 m NaOH. Add the COM drop-out powder and the agar (if used). Adjust the volume to 1 L. Autoclave at 0.5 bar for 20 min at 121°C. Store for several weeks at room temperature.
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  1. doi:10.1101/pdb.rec090514 Cold Spring Harb Protoc 2017: pdb.rec090514- © 2017 Cold Spring Harbor Laboratory Press
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