cDNA Synthesis Buffer
| Reagent | Quantity (for 20 µL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.3) | 1.0 µL | 50 mm |
| MgCl2 (0.6 m) | 0.2 µL | 6 mm |
| MnCl2 (0.2 m) | 0.2 µL | 2 mm |
| dNTP solution (each of the four dNTPs at 10 mm; pH 8.0) | 2.0 µL | 1 mm each |
| Dithiothreitol (1 m) | 0.2 µL | 10 mm |
| Bovine serum albumin (2 mg/mL) | 2.0 µL | 0.2 mg/mL |
| CapFinder primer (1 nm) | 0.2 µL | 10 pm |
| Template mRNA (on washed beads) | 1–10 µL of mRNA bound to beads | |
| H2O | To a final volume of 20 µL |
