Synthetic Minimal Solid Medium
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1. In a 0.5-L bottle, combine 200 mL of H2O and 10 g of agar. In a 250-mL bottle, combine 3.4 g of yeast nitrogen base (YNB) without amino acids (Sigma-Aldrich Y0626) and 200 mL of H2O. Autoclave each solution for 30 min at 15 psi and then combine.
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It is critical to autoclave agar and YNB separately, as the low pH of YNB will hydrolyze the agar and yield semisolid plates.
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2. Add 25 mL of 40% (w/v) glucose (separately sterilized by autoclaving).
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3. Pour plates as required for use.
