Recipe

Enzyme Solution for Adult Tissue Dissociation

  • 1. To prepare EnzymeMix1 of the Neural Tissue Dissociation Kit (Miltenyi Biotec, catalog no. 130-092-628), combine 50 µL of Solution 1 with 1900 µL of Solution 2.

  • 2. Vortex and incubate it (37°C, 20 min) to get EnzymeMix1.

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  1. doi:10.1101/pdb.rec095364 Cold Spring Harb Protoc 2017: pdb.rec095364- © 2017 Cold Spring Harbor Laboratory Press

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