Protocol

Transformation of Schizosaccharomyces pombe in a 96-Well Format

  1. Nevan J. Krogan
  1. Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518
  1. ↵1Correspondence: roguev{at}gmail.com

Abstract

This protocol describes chemical transformation of Schizosaccharomyces pombe with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast.

Footnotes

  • From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.

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  1. Published in Advance July 21, 2017, doi: 10.1101/pdb.prot091942 Cold Spring Harb Protoc 2018: pdb.prot091942- © 2018 Cold Spring Harbor Laboratory Press
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