Protocol

DNA Preparation from Schizosaccharomyces pombe

  1. Nevan J. Krogan
  1. Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518
  1. ↵1Correspondence: roguev{at}gmail.com

Abstract

This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

Footnotes

  • From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.

Articles citing this article

  • Genetic Interaction Mapping in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a ROTOR HDA Colony Replicating Robot in a 1536 Array Format Cold Spring Harb Protoc; 2018;
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This Article

  1. Published in Advance July 21, 2017, doi: 10.1101/pdb.prot091959 Cold Spring Harb Protoc 2018: pdb.prot091959- © 2018 Cold Spring Harbor Laboratory Press
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