Embryonic Immunostaining for the Tardigrade Hypsibius exemplaris
- 1Biology Department, University of North Florida, Jacksonville, Florida 32224;
- 2Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
- ↵3Correspondence: frank.smith{at}unf.edu
Abstract
Immunostaining is a method used to visualize the localization of proteins in fixed tissue. Many antibodies are available that recognize specific proteins in a wide diversity of organisms, which makes this method ideal for investigating gene expression patterns in nonmodel animal systems. This protocol describes immunostaining for studies of embryogenesis in the tardigrade Hypsibius exemplaris.
Footnotes
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From the Emerging Model Organisms collection.
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Supplemental material is available for this article at cshprotocols.cshlp.org.
