Recipe

PBT (0.5×)

Phosphate-buffered saline (PBS; 10X, pH 7.4) 2.5 mL
Triton X-100 (10% [v/v] in DEPC-treated H2O) 500 µL
Distilled H2O  47 mL
Final volume  50 mL
Store for up to 1 mo at room temperature.
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  1. doi:10.1101/pdb.rec102517 Cold Spring Harb Protoc 2018: pdb.rec102517- © 2018 Cold Spring Harbor Laboratory Press

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