LysoTracker Green DND-26 Staining Solution (75 nm)

• 1. Allow a vial of 1 mm LysoTracker Green DND-26 solution (50 µL in DMSO) (Invitrogen L7526) to warm to room temperature. Briefly centrifuge to deposit the solution at the bottom of the vial before opening.

• 2. From the 1 mm solution, prepare a 500 nm stock solution using molecular-grade H₂O. Cover in aluminum foil to minimize light exposure. Store at or below −20°C.

• 3. Immediately before use, prepare a staining solution of roughly 75 nm Lysotracker Green by combining 2 µL of the 500 nm stock solution with 10 µL of spring water.

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